Analysis of LT and ST Genes of Enterotoxigenic Escherichia coli Using Polymerase Chain Reaction Method in Lawar Plek from Ketewel Village, Gianyar
DOI:
https://doi.org/10.53770/medica.v6i2.504Keywords:
Enterotoxigenic, Escherichia coli, Lawar Plek, Polymerase Chain ReactionAbstract
Lawar plek is a traditional Balinese dish made from raw meat, which poses a potential risk of contamination by pathogenic bacteria, including Enterotoxigenic Escherichia coli (ETEC). This study aimed to identify the presence of ETEC in lawar plek samples from Ketewel Village, Gianyar. This research uses molecular methods. The identification was conducted in two stages: initial isolation of suspected bacteria on Eosin Methylene Blue Agar (EMBA), followed by molecular confirmation using Polymerase Chain Reaction (PCR). The study results show that in EMBA culture, 4 out of 12 samples (33.33%) showed colonies presumptively identified as E. coli. However, PCR analysis revealed that none of these isolates carried the LT (heat-labile toxin) or ST (heat-stable toxin) genes characteristic of ETEC. The conclusion shows that neither the LT (heat-labile toxin) nor the ST (heat-stable toxin) virulence genes of Enterotoxigenic Escherichia coli were found in any of the samples tested as suspected Escherichia coli colonies on EMBA.
References
Abri, R., Javadi, A., Asghari, R., Razavilar, V., Salehi, T. Z., Safaeeyan, F., & Rezaee, M. A. (2019). Surveillance for enterotoxigenic & enteropathogenic Escherichia coli isolates from animal source foods in Northwest Iran. Indian Journal of Medical Research, 150(1), 87-91.
Adiputra, S., Trisnadewi, N. W., Putu, N., Oktaviani, W., Munthe, S.a., …..& Suryana, S. (2021). Metodologi Penelitian Kesehatan. Medan:Yayasan Kita Menulis.
Apsari, P. I. B., Suryanditha, P. A., Widhidewi, N. W., & Masyeni, D. A. P. S. (2023). Characteristics of the Health Problems Among International Travelers Visiting International Hospital in Bali. WMJ (Warmadewa Medical Journal), 8(1), 23-26. https://doi.org/10.22225/wmj.8.1.5869.23-26
Badan Penelitian dan Pengembangan Kesehatan. (2019). Laporan Nasional RISKESDAS 2018. Jakarta: Badan Penelitian dan Pengembangan Kesehatan.
Badan Pusat Statistik Provinsi Bali. (2023). Banyaknya Wisatawan Mancanegara ke Bali dan Indonesia, 1969-2024. Badan Pusat Statistik Provinsi Bali.
Ballout, R., Toufeili, I., Kharroubi, S. A., & Kassem, I. I. (2023). Raw meat consumption and food safety challenges: a survey of knowledge, attitudes, and practices of consumers in Lebanon. Foods, 13(1), 118. https://doi.org/10.3390/foods13010118
Dinas Kesehatan Provinsi Bali. (2017). Profil Kesehatan Provinsi Bali Tahun 2017. Denpasar: Dinas Kesehatan Provinsi Bali.
Dinas Kesehatan Kabupaten Gianyar. (2019). Profil Kesehatan Kabupaten Gianyar 2019. Gianyar: Dinas Kesehatan Kabupaten Gianyar.
Ekawati, E. R., Yusmiati, S. N. H., & Hamidi, F. R. (2017). Deteksi Escherichia coli patogen pada pangan menggunakan metode konvensional dan metode multiplex PCR. Jurnal SainHealth, 1(2), 75-82. https://doi.org/10.51804/jsh.v1i2.107.75-82
Fatiqin, A., Novita, R., & Apriani, I. (2019). Pengujian Salmonella dengan menggunakan media ssa dan E. coli menggunakan media EMBA pada bahan pangan. Indobiosains,1(1):22-29. https://doi.org/10.31851/indobiosains. v1i1.2206
Gomes, T. A., Elias, W. P., Scaletsky, I. C., Guth, B. E., Rodrigues, J. F., Piazza, R. M., ... & Martinez, M. B. (2016). Diarrheagenic escherichia coli. Brazilian journal of microbiology, 47(Suppl. 1), 3-30. https://doi.org/10.1016/j.bjm.2016.10.015
Hutami, R., Bisyri, H., Sukarno, S., Nuraini, H., & Ranasasmita, R. (2018). Ekstraksi DNA dari daging segar untuk analisis dengan metode loop-mediated isothermal amplification (LAMP). Jurnal Agroindustri Halal, 4(2), 209-216. https://doi.org/10.30997/jah.v4i2.1409
Jamilatun, M., & Aminah, A. (2016). Isolasi dan identifikasi Escherichia coli pada air wudhu di masjid yang berada di Kota Tangerang. Jurnal Medikes (Media Informasi Kesehatan), 3(1), 81-90. https://doi.org/10.36743/medikes.v3i1.154
Javadi, A., Shamaei, M., Ziazi, L. M., Pourabdollah, M., Dorudinia, A., Seyedmehdi, S. M., & Karimi, S. (2014). Qualification study of two genomic DNA extraction methods in different clinical samples. Tanaffos, 13(4),41-7.
Jyoti, A., Agarwal, M., & Tomar, R. S. (2015). Culture-Free Detection of Enterotoxigenic Escherichia coli in Food by Polymerase Chain Reaction. International Journal for Research in Applied Science & Engineering Technology, 3(4), 1060-1064.
Kementerian Kesehatan Republik Indonesia. (2015) Profil Kesehatan Indonesia Tahun 2015. Jakarta: Kementerian Kesehatan Republik Indonesia.
Khakim, L., & Rini, C. S. (2018). Identifikasi Eschericia coli dan Salmonella sp. pada air kolam renang candi pari. Medicra (Journal of Medical Laboratory Science/Technology), 1(2), 84-93. http://doi.org/10.21070/medicra.v1i2.1491
Masyeni, D. A. P. S., Sukmawati, H., Paramasatiari, L., Aryastuti, S. A., Somia, K. A., Kambayana, G., ... & Merati, T. P. (2017). Diarrhea Among International Travelers in BaliIndonesia: Clinical and Microbiological Finding. International Journal of Travel Medicine and Global Health. International Journal of Travel Medicine and Global Health, 5(3), 84-88.
Mohammadzadeh, M., Goudarzi, H., Dabiri, H., & Fallah, F. (2015). Distribution of Enterotoxigenic Escherichia coli among E. coli isolates from diarrheal samples referred to educational hospitals in Tehran-Iran. Novelty in Biomedicine, 3(3), 144-147. https://doi.org/10.22037/nbm.v3i3.8533
Nalendra, A. R. A., Rosalinah, Y., Priadi, A., Subroto, I., Rahayuningsih, R., Lestari, R., ... & Zede, V. A. (2021). Statistika Seri Dasar dengan SPSS. Bandung: Media Sains Indonesia.
Nugraha, F., Roslim, D. I., & Ardilla, Y. P. (2014). Analisis sebagian sekuen gen Ferritin2 pada padi (Oryza sativa L.) Indragiri Hilir, Riau. Biosaintifika: Journal of Biology & Biology Education, 6(2), 70-79. https://doi.org/10.15294/biosaintifika.v6i2.3102
Novitasari, D. A., Elvyra, R., & Roslim, D. I. (2014). Teknik Isolasi Dan Elektroforesis DNA Total Pada Kryptopterus apogon (Bleeker 1851) dari Sungai Kampar Kiri Dan Tapung Hilir Kabupaten Kampar Provinsi Riau. Jurnal Online Mahasiswa, Fakultas Matematika dan Ilmu Pengetahuan Alam, 1(2):258-61.
Purnama, S. G., Purnama, H., & Subrata, I. M. (2017). Kualitas Mikrobiologis Dan Higiene Pedagang Lawar Di Kawasan Pariwisata Kabupaten Gianyar. Jurnal Kesehatan Lingkungan Indonesia, 16(2), 56-62. https://doi.org/10.14710/jkli.16.2.56-62
Purwanata, H., & Ekayani, I. A. P. H. (2023). Persepsi Konsumen Pada Produk Lawar Plek di Desa Ketewel Kabupaten Gianyar. Jurnal Kuliner, 3(1), 1-10. http://10.23887/jk.v3i1.60030
Putra, L. A. G., Yonathan, C. J., Niedhatrata, N. I., Firdaus, M. H. R., & Yoewono, J. R. (2020). A review of the development of Polymerase Chain Reaction technique and its uses in Scientific field. Stannum: Jurnal Sains dan Terapan Kimia, 2(1), 14-30.
Qamariya, K., Sulihingtyas, W. D., & Wirajana, I. N. (2016). Alternatif Penggunaan Silika Bentonit Sebagai Pengganti Fenol-Kloroform-Isoamil Alkohol Dalam Ekstraksi Dna Secara Langsung Dari Tanah Hutan Mangrove. Jurnal Kimia. 10(1); 43-48. https://doi.org/10.24843/JCHEM.2016.v10.i01.p06
Soeprapto, V. S., & Yohana, E. (2021). Eksplorasi Wisata Kuliner-Studi Kasus Di Bali. Journal FAME: Journal Food and Beverage, Product and Services, Accomodation Industry, Entertainment Services, 4(1), 46-52. http://dx.doi.org/10.30813/fame.v4i1.2
Sophian, A., & Yustina, Y. (2022). Analisis nilai kemurnian DNA menggunakan Nano fotometer pada rasio 260/230 yang diisolasi dari produk nugget. Muhammadiyah Journal of Nutrition and Food Science (MJNF), 3(2), 82-86. https://doi.org/10.24853/mjnf.3.2.82-86
Suastina, G. A. P. D., Arjita, I. P. D., & Adnyana, I. G. A. (2023). Efek base genep terhadap total plate count dan most probable number Escherichia coli pada lawar plek. Intisari Sains Medis, 14(1), 504-512. https://doi.org/10.15562/ism.v14i1.1654
Sukrama, I. D. M., Lestari, A. A. W., Hendrayana, M. A., & Somia, I. K. A. (2020). Antibiotic Resistance Pattern and Detection of Enterotoxigenic and Enteroaggregative Strain of Escherichia coli among Foreign Tourist with Traveler Diarrhea in Bali using Uniplex Polymerase Chain Reaction. Open Access Macedonian Journal of Medical Sciences, 8(A), 583-588. https://doi.org/10.3889/oamjms.2020.4863
Tilawah, S., Sari, R, & Apridamayanti, P. (2019). Optimasi Volume Dna Marker Dan Volume Dna Hasil Amplifikasi Gen Tetl Resistensi Antibiotik Tetrasiklin Dari Bakteri Bacillus cereus Pada Pasien Ulkus Diabetik. Jurnal Mahasiswa Farmasi Fakultas Kedokteran Untan, 4(1).
Trisno, K., Tono, K. P., & Suarjana, I. G. K. (2019). Isolasi dan indentifikasi bakteri Escherichia coli dari udara pada rumah potong unggas swasta di Kota Denpasar. Indonesia Medicus Veterinus, 8(5), 685-694.
Yuda, I. W. E. D., Nityasa, N. P. N., & Lestari, A. P. U. P. (2019). Pusat Kuliner Tradisional Khas Bali Di Kabupaten Gianyar. Jurnal Teknik Gradien, 11(1), 57-63.
World Health Organization. (2019). Foodborne diseases: Global burden. World Health Organization. Foodborne diseases. Retrieved from: https://www.who.int/news-room/questions-and-answers/item/foodborne-diseases-global-burden
World Health Organization. (2024). Diarrhoeal Disease. World Health Organization Retrieved from: https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease
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