Impact of Sample Delay on Leukocyte and Platelet Parameters in Dengue Fever Patients: A Hematological Analysis

Luh Mitha Ari Utami; Heri Setiyo Bekti; I Gusti Ayu Sri Dhyanaputri

doi:10.53770/medica.v6i2.502

Authors

Luh Mitha Ari Utami Department of Medical Laboratory Technology, Politeknik Kesehatan Kementerian Kesehatan Denpasar, Denpasar, Bali, Indonesia
Heri Setiyo Bekti Department of Medical Laboratory Technology, Politeknik Kesehatan Kementerian Kesehatan Denpasar, Denpasar, Bali, Indonesia
I Gusti Ayu Sri Dhyanaputri Department of Medical Laboratory Technology, Politeknik Kesehatan Kementerian Kesehatan Denpasar, Denpasar, Bali, Indonesia

DOI:

https://doi.org/10.53770/medica.v6i2.502

Keywords:

Dengue Hemorrhagic Fever, Leukocyte Count, Platelet Count, Delayed Examination

Abstract

Background: The diagnosis of Dengue Fever (DF) involves physical examination, laboratory tests, and confirmation primarily through thrombocytopenia and leukocyte abnormalities. Laboratory procedures play a crucial role; however, delays in complete blood count (CBC) analysis may influence hematological parameters, potentially affecting diagnostic accuracy.

Objective: To determine the effect of 2-hour and 4-hour delays in CBC testing on leukocyte and platelet counts in patients with Dengue Fever. Methods: This experimental study used a posttest-only control group design with purposive sampling. Blood samples from DF patients were divided into three groups: immediate testing, 2-hour delay, and 4-hour delay. Data were analyzed using the Shapiro-Wilk test followed by Multivariate Analysis of Variance (MANOVA). Results: The mean leukocyte and platelet counts in the immediate testing group were 4.86×10³/μL and 61.55×10³/μL, respectively. In the 2-hour delay group, leukocyte counts slightly decreased to 4.79×10³/μL and platelet counts increased to 65.77×10³/μL. In the 4-hour delay group, leukocyte counts increased to 4.85×10³/μL while platelet counts decreased to 64×10³/μL. MANOVA results (Sig. = 1.000) indicated no significant differences among the groups. Conclusion: Delays of 2 and 4 hours in CBC testing did not significantly affect leukocyte and platelet counts in Dengue Fever patients. These findings suggest that short-term delays in sample analysis may not compromise hematological results in DF diagnosis.

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